Title

A mechanism for Ikaros regulation of human globin gene switching

UMMS Affiliation

Program of Gene Function and Expression

Date

5-2008

Document Type

Article

Medical Subject Headings

Animals; Chromatin; DNA-Binding Proteins; Fetal Hemoglobin; Gene Expression Regulation, Developmental; *Genes, Switch; Globins; Humans; Ikaros Transcription Factor; Mice; Mice, Inbred C57BL; Mice, Transgenic; Point Mutation; Reverse Transcriptase Polymerase Chain Reaction

Disciplines

Genetics and Genomics

Abstract

The human beta globin locus consists of an upstream LCR and functional genes arranged sequentially in the order of their expression during development: 5'-HBE1, HBG2, HBG1, HBD, HBB-3'. Haemoglobin switching entails the successive recruitment of these genes into an active chromatin hub (ACH). Here we show that the transcription factor Ikaros plays a major role in the formation of the beta-globin ACH, and in haemoglobin switching. In Plastic mice, where the DNA-binding region of Ikaros is disrupted by a point mutation, there is concomitant marked down-regulation of HBB, and up-regulation of HBG expression. We show for the first time Ikaros and its family member Eos, bind to critical cis elements implicated in haemoglobin switching and deletional hereditary persistence of fetal haemoglobin (HPFH). Chromatin conformation capture (3C) data demonstrated that Ikaros facilitates long-distance DNA looping between the LCR and a region upstream of HBD. This study provides new insights into the mechanism of stage-specific assembly of the beta-globin ACH, and HPFH.

Rights and Permissions

Citation: Br J Haematol. 2008 May;141(3):398-406. Epub 2008 Mar 3. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

18318763