C. elegans sirtuins
Program in Gene Function and Expression; Program in Molecular Medicine
Medical Subject Headings
Adipocytes; Animals; Animals, Genetically Modified; Caenorhabditis elegans; Humans; Longevity; Oxidative Stress; Sirtuins
Biochemistry | Computational Biology | Genetics and Genomics | Molecular Biology
The nematode Caenorhabditis elegans (C. elegans) has four Sir2 paralogs, sir-2.1, sir-2.2, sir-2.3, and sir-2.4. Thus far, most of the research tools to study worm sirtuins have been developed for sir-2.1, due to its homology to yeast SIR2 and human SIRT1. Here, we have compiled a listing of the currently available strains (including both loss-of-function alleles and transgenics), antibodies, and RNAi constructs relevant to studies on all C. elegans sirtuin family members. We also describe the methods used in the analysis of C. elegans sirtuin function, including life span analysis, various stress-resistance assays, and fat content analysis and provide an overview of all phenotypic data relevant to C. elegans sir-2.1.
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Citation: Viswanathan M, Tissenbaum HA. C. elegans sirtuins. Methods Mol Biol. 2013;1077:39-56. doi: 10.1007/978-1-62703-637-5_3. Link to article on publisher's site
Viswanathan, Mohan and Tissenbaum, Heidi A., "C. elegans sirtuins" (2013). Program in Gene Function and Expression Publications and Presentations. 244.