UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine

Date

10-19-2004

Document Type

Article

Medical Subject Headings

Animals; Animals, Genetically Modified; Caenorhabditis elegans; Cloning, Molecular; Gene Expression; *Genes, Helminth; Green Fluorescent Proteins; Luminescent Proteins; Open Reading Frames; Promoter Regions, Genetic; Transcription Factors

Disciplines

Genetics and Genomics

Abstract

An important aspect of the development of systems biology approaches in metazoans is the characterization of expression patterns of nearly all genes predicted from genome sequences. Such "localizome" maps should provide information on where (in what cells or tissues) and when (at what stage of development or under what conditions) genes are expressed. They should also indicate in what cellular compartments the corresponding proteins are localized. Caenorhabditis elegans is particularly suited for the development of a localizome map since all its 959 adult somatic cells can be visualized by microscopy, and its cell lineage has been completely described. Here we address one of the challenges of C. elegans localizome mapping projects: that of obtaining a genome-wide resource of C. elegans promoters needed to generate transgenic animals expressing localization markers such as the green fluorescent protein (GFP). To ensure high flexibility for future uses, we utilized the newly developed MultiSite Gateway system. We generated and validated "version 1.1" of the Promoterome: a resource of approximately 6000 C. elegans promoters. These promoters can be transferred easily into various Gateway Destination vectors to drive expression of markers such as GFP, alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).

Rights and Permissions

Citation: Genome Res. 2004 Oct;14(10B):2169-75. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

15489340

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