Title

An optimized two-finger archive for ZFN-mediated gene targeting

UMMS Affiliation

Program in Gene Function and Expression; Department of Biochemistry and Molecular Pharmacology

Date

4-29-2012

Document Type

Article

Medical Subject Headings

Zinc Fingers; Gene Targeting

Disciplines

Genetics and Genomics

Abstract

The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.

Rights and Permissions

Citation: Nat Methods. 2012 Apr 29. doi: 10.1038/nmeth.1994. [Epub ahead of print]

Related Resources

Link to article in PubMed

PubMed ID

22543349