Title
An optimized two-finger archive for ZFN-mediated gene targeting
UMMS Affiliation
Program in Gene Function and Expression; Department of Biochemistry and Molecular Pharmacology
Date
4-29-2012
Document Type
Article
Medical Subject Headings
Zinc Fingers; Gene Targeting
Disciplines
Genetics and Genomics
Abstract
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
Rights and Permissions
Citation: Nat Methods. 2012 Apr 29. doi: 10.1038/nmeth.1994. [Epub ahead of print]
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