Title

Mbd3/NURD complex regulates expression of 5-hydroxymethylcytosine marked genes in embryonic stem cells

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology; Program in Gene Function and Expression; Program in Molecular Medicine

Date

12-23-2011

Document Type

Article

Medical Subject Headings

Animals; Chromatin Assembly and Disassembly; Cytosine; DNA Helicases; DNA-Binding Proteins; Embryonic Stem Cells; Gene Knockdown Techniques; Humans; Mi-2 Nucleosome Remodeling and Deacetylase Complex; Mice; Nuclear Proteins; Proto-Oncogene Proteins; RNA Polymerase II; Transcription Factors

Disciplines

Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics

Abstract

Numerous chromatin regulators are required for embryonic stem (ES) cell self-renewal and pluripotency, but few have been studied in detail. Here, we examine the roles of several chromatin regulators whose loss affects the pluripotent state of ES cells. We find that Mbd3 and Brg1 antagonistically regulate a common set of genes by regulating promoter nucleosome occupancy. Furthermore, both Mbd3 and Brg1 play key roles in the biology of 5-hydroxymethylcytosine (5hmC): Mbd3 colocalizes with Tet1 and 5hmC in vivo, Mbd3 knockdown preferentially affects expression of 5hmC-marked genes, Mbd3 localization is Tet1-dependent, and Mbd3 preferentially binds to 5hmC relative to 5-methylcytosine in vitro. Finally, both Mbd3 and Brg1 are themselves required for normal levels of 5hmC in vivo. Together, our results identify an effector for 5hmC, and reveal that control of gene expression by antagonistic chromatin regulators is a surprisingly common regulatory strategy in ES cells.

Rights and Permissions

Citation: Cell. 2011 Dec 23;147(7):1498-510. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

22196727