Title

Ceramidase expression facilitates membrane turnover and endocytosis of rhodopsin in photoreceptors

UMMS Affiliation

Program in Gene Function and Expression

Date

2-11-2004

Document Type

Article

Medical Subject Headings

Amidohydrolases; Animals; Animals, Genetically Modified; Cell Membrane; Ceramidases; Darkness; Drosophila Proteins; Drosophila melanogaster; *Endocytosis; Eye Proteins; Half-Life; Ligands; Light; Mutation; Photoreceptor Cells, Invertebrate; Receptors, G-Protein-Coupled; Retinal Degeneration; Rhodopsin

Disciplines

Genetics and Genomics

Abstract

Transgenic expression of ceramidase suppresses retinal degeneration in Drosophila arrestin and phospholipase C mutants. Here, we show that expression of ceramidase facilitates the dissolution of incompletely formed and inappropriately located elements of rhabdomeric membranes in ninaE(I17) mutants lacking the G protein receptor Rh1 in R1-R6 photoreceptor cells. Ceramidase expression facilitates the endocytic turnover of Rh1. Although ceramidase expression aids the removal of internalized rhodopsin, it does not affect the turnover of Rh1 in photoreceptors maintained in dark, where Rh1 is not activated and thus has a slower turnover and a long half-life. Therefore, the phenotypic consequence of ceramidase expression in photoreceptors is caused by facilitation of endocytosis. This study provides mechanistic insight into the sphingolipid biosynthetic pathway-mediated modulation of endocytosis and suppression of retinal degeneration.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1922-6. Epub 2004 Feb 9. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

14769922