Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C
Program in Gene Function and Expression
Medical Subject Headings
Cell Cycle Proteins; *DNA Replication; Histones; Protein Binding; S Phase
Genetics and Genomics
Chromatin assembly and DNA replication are temporally coupled, and DNA replication in the absence of histone synthesis causes inviability. Here we demonstrate that chromatin assembly factor Asf1 also affects DNA replication. In budding yeast cells lacking Asf1, the amounts of several DNA replication proteins, including replication factor C (RFC), proliferating cell nuclear antigen (PCNA), and DNA polymerase epsilon (Pol epsilon), are reduced at stalled replication forks. In contrast, DNA polymerase alpha (Pol alpha) accumulates to higher than normal levels at stalled forks in asf1Delta cells. Using purified, recombinant proteins, we demonstrate that RFC directly binds Asf1 and can recruit Asf1 to DNA molecules in vitro. We conclude that histone chaperone protein Asf1 maintains a subset of replication elongation factors at stalled replication forks and directly interacts with the replication machinery.
Rights and Permissions
Citation: Genes Dev. 2005 Jun 1;19(11):1365-75. Epub 2005 May 18. Link to article on publisher's site