XBP1 activates the transcription of its target genes via an ACGT core sequence under ER stress
Program in Gene Function and Expression; Program in Molecular Medicine
Medical Subject Headings
Base Sequence; DNA; DNA-Binding Proteins; Endoplasmic Reticulum; Hela Cells; Humans; Molecular Sequence Data; Nuclear Proteins; Promoter Regions, Genetic; Transcription Factors; Transcription, Genetic
Genetics and Genomics
In mammals, the transmembrane protein kinase/endoribonuclease IRE1 is activated by endoplasmic reticulum stress and subsequently processes XBP1 mRNA to generate an active form of XBP1 protein. The spliced form of XBP1 protein acts as a transcription factor and induces the expression of ER-resident molecular chaperones. However, the mechanism for how XBP1 promotes the transcription of its target genes as well as the cis-acting elements for XBP1 during ER stress has been unclear. Recently, it was demonstrated that the expression of MDG1/ERdj4, a member of the DnaJ family, is regulated by the IRE1-XBP1 pathway. In the present report, we investigated the regulatory mechanisms of MDG1/ERdj4 gene expression by XBP1. We identified a cis-acting element in the MDG1/ERdj4 promoter region, to which XBP1 specifically binds in response to ER stress. Our results reveal a target sequence for the IRE1-XBP1 pathway under ER stress conditions.
Rights and Permissions
Citation: Biochem Biophys Res Commun. 2005 Jun 17;331(4):1146-53. Link to article on publisher's site
Kanemoto, Soshi; Kondo, Shinichi; Ogata, Maiko; Murakami, Tomohiko; Urano, Fumihiko; and Imaizumi, Kazunori, "XBP1 activates the transcription of its target genes via an ACGT core sequence under ER stress" (2005). Program in Gene Function and Expression Publications and Presentations. Paper 130.