Program in Gene Function and Expression; Program in Molecular Medicine
Medical Subject Headings
Caenorhabditis elegans; Caenorhabditis elegans Proteins; Protein Interaction Mapping; Two-Hybrid System Techniques; Tandem Mass Spectrometry
Genetics and Genomics
Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).