Program in Gene Function and Expression; Program in Molecular Medicine
Medical Subject Headings
Caenorhabditis elegans; Caenorhabditis elegans Proteins; Protein Interaction Mapping; Two-Hybrid System Techniques; Tandem Mass Spectrometry
Genetics and Genomics
Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).
Rights and Permissions
Walhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook, http://dx.doi.org/110.1895/wormbook.1.86.1, http://www.wormbook.org.
Walhout, Albertha J. M. and Boulton, Simon J., "Biochemistry and Molecular Biology" (2006). Program in Gene Function and Expression Publications and Presentations. 1.