UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine

Date

2-13-2006

Document Type

Book Chapter

Medical Subject Headings

Caenorhabditis elegans; Caenorhabditis elegans Proteins; Protein Interaction Mapping; Two-Hybrid System Techniques; Tandem Mass Spectrometry

Disciplines

Genetics and Genomics

Abstract

Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).

Rights and Permissions

Walhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook, http://dx.doi.org/110.1895/wormbook.1.86.1, http://www.wormbook.org.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.