UMMS Affiliation
Program in Gene Function and Expression; Program in Molecular Medicine
Date
2-13-2006
Document Type
Book Chapter
Medical Subject Headings
Caenorhabditis elegans; Caenorhabditis elegans Proteins; Protein Interaction Mapping; Two-Hybrid System Techniques; Tandem Mass Spectrometry
Disciplines
Genetics and Genomics
Abstract
Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).
Rights and Permissions
Walhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook, http://dx.doi.org/110.1895/wormbook.1.86.1, http://www.wormbook.org.