Document Type
Book ChapterPublication Date
2006-02-13Keywords
Caenorhabditis elegansCaenorhabditis elegans Proteins
Protein Interaction Mapping
Two-Hybrid System Techniques
Tandem Mass Spectrometry
Genetics and Genomics
Metadata
Show full item recordAbstract
Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).Source
Walhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook, http://dx.doi.org/110.1895/wormbook.1.86.1, http://www.wormbook.org.DOI
110.1895/wormbook.1.86.1Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43888ae974a485f413a2113503eed53cd6c53
110.1895/wormbook.1.86.1