Title

Engineered telomeres in transgenic Xenopus laevis

UMMS Affiliation

Department of Cell and Developmental Biology

Date

6-1-2007

Document Type

Article

Medical Subject Headings

Animals; Animals, Genetically Modified; DNA; *Epigenesis, Genetic; Genetic Engineering; In Situ Hybridization, Fluorescence; Models, Animal; Telomere; Xenopus laevis

Disciplines

Cell Biology | Developmental Biology | Molecular Biology | Molecular Genetics

Abstract

The expanding roles of telomeres in epigenetic gene regulation, nuclear organization, and human disease have necessitated the establishment of model organisms in which to study telomere function under normal developmental conditions. We present an efficient system for generating numerous vertebrate animals containing engineered telomeres using a Xenopus laevis transgenesis technique. Our results indicate Xenopus zygotes efficiently recognize telomeric repeats at chromosome break points and form telomeric complexes thus generating a new telomere. The resulting transgenic animals progress through normal development and successfully metamorphose into froglets despite the chromosome breakage. Overall, this presents an efficient mechanism for generating engineered telomeres in a vertebrate system and provides an opportunity to investigate epigenetic aspects of telomere function during normal vertebrate development.

Rights and Permissions

Citation: Transgenic Res. 2007 Jun;16(3):377-84.doi: 10.1007/s11248-007-9076-0. Link to article on publisher's site

Comments

At the time of publication, Peter Jones was not yet affiliated with the University of Massachusetts Medical School.

Related Resources

Link to Article in PubMed

Keywords

Telomere, Transgenic Xenopus, laevis

PubMed ID

17447124