Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing
Department of Cell and Developmental Biology
Medical Subject Headings
Animals; Cell Cycle Proteins; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Drosophila Proteins; Fragile X Mental Retardation Protein; Gene Silencing; Histones; Humans; Methyl-CpG-Binding Protein 2; Mice; Microscopy, Fluorescence; Molecular Sequence Data; NIH 3T3 Cells; Nerve Tissue Proteins; RNA-Binding Proteins; Repressor Proteins; Trans-Activators; Transcription Factors
Cell Biology | Developmental Biology | Molecular Biology | Molecular Genetics
Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.
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Citation: Nat Genet. 2005 Mar;37(3):254-64. Epub 2005 Feb 6. Link to article on publisher's site
Harikrishnan, K. N.; Chow, Maggie Z.; Baker, Emma K.; Pal, Sharmistha; Bassal, Sahar; Brasacchio, Daniella; Wang, Li; Craig, Jeff M.; Jones, Peter L.; Sif, Said; and El-Osta, Assam, "Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing" (2005). Peter Jones Lab Publications. 4.