Title

Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing

UMMS Affiliation

Department of Cell and Developmental Biology

Date

3-1-2005

Document Type

Article

Medical Subject Headings

Animals; Cell Cycle Proteins; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Drosophila Proteins; Fragile X Mental Retardation Protein; Gene Silencing; Histones; Humans; Methyl-CpG-Binding Protein 2; Mice; Microscopy, Fluorescence; Molecular Sequence Data; NIH 3T3 Cells; Nerve Tissue Proteins; RNA-Binding Proteins; Repressor Proteins; Trans-Activators; Transcription Factors

Disciplines

Cell Biology | Developmental Biology | Molecular Biology | Molecular Genetics

Abstract

Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.

Rights and Permissions

Citation: Nat Genet. 2005 Mar;37(3):254-64. Epub 2005 Feb 6. Link to article on publisher's site

Comments

At the time of publication, Peter Jones was not yet affiliated with the University of Massachusetts Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

15696166