Title

Potential utility of plasma fatty acid analysis in the diagnosis of cystic fibrosis

UMMS Affiliation

Department of Pediatrics

Date

1-30-2007

Document Type

Article

Medical Subject Headings

Adolescent; Adult; Cystic Fibrosis; Fatty Acids; Female; Gas Chromatography-Mass Spectrometry; Humans; Male; Plasma

Disciplines

Allergy and Immunology | Pediatrics | Respiratory Tract Diseases

Abstract

BACKGROUND: An altered distribution of fatty acids in cells and tissues is found in patients with cystic fibrosis (CF). In this study, we assessed the potential role of plasma fatty acid analysis in the diagnosis of CF.

METHODS: In this 2-part study, we first used gas chromatography-mass spectrometry to analyze fatty acids in plasma from 13 CF patients and 11 controls without CF. We then used the fatty acid distribution data to identify the fatty acids or multiple fatty acid calculations most effective in identifying CF patients. Part 2 of the study was a blinded analysis of 10 CF patients and 9 controls to directly test the effectiveness of the diagnostic parameters for CF identified from the plasma fatty acid analysis.

RESULTS: In the nonblinded trial, the multiplication product of (18:2 n-6) x (22:6 n-3) (each as percentage of total plasma fatty acid) was the most effective indicator for distinguishing patients with CF from controls (P = 0.0003). In part 2 (the blinded trial), this multiplication product was also the most effective indicator for distinguishing CF patients from controls (P = 0.0008).

CONCLUSIONS: The product of (18:2 n-6) x (22:6 n-3) is effective for distinguishing CF patients from persons without CF. This diagnostic marker may have value as an alternative to the sweat chloride test in selected patients being evaluated for CF.

Rights and Permissions

Citation: Clin Chem. 2007 Jan;53(1):78-84. Epub 2006 Nov 27. Link to article on publisher's site

Related Resources

Link to Article in PubMed