Title

Lack of telomere shortening with age in mouse resting zone chondrocytes

UMMS Affiliation

Department of Pediatrics, Division of Endocrinology

Date

3-24-2005

Document Type

Article

Medical Subject Headings

Aging; Animals; Blotting, Southern; Cell Division; Chondrocytes; Growth Plate; Mice; Telomere

Disciplines

Pediatrics

Abstract

BACKGROUND AND AIM: Telomeres are hexameric repeat sequences that flank eukaryotic chromosomes. The telomere hypothesis of cellular aging proposes that replication of normal somatic cells leads to progressive telomere shortening which induces replicative senescence. Previous studies suggest that growth plate chondrocytes have a finite proliferative capacity in vivo. We therefore hypothesized that telomere shortening in resting zone chondrocytes leads to replicative senescence.

METHOD: To test this hypothesis we compared the telomere restriction fragment (TRF) length of Mus casteneus at 1, 4, 8, and 56 weeks of age.

RESULTS AND CONCLUSIONS: We found that TRF length did not diminish measurably with age, suggesting that telomere shortening in resting zone chondrocytes is not the mechanism that limits proliferation of growth plate chondrocytes in vivo.

Rights and Permissions

Citation: Horm Res. 2005;63(3):125-8. Epub 2005 Mar 24. Link to article on publisher's site

Related Resources

Link to Article in PubMed