Lack of telomere shortening with age in mouse resting zone chondrocytes
Department of Pediatrics, Division of Endocrinology
Medical Subject Headings
Aging; Animals; Blotting, Southern; Cell Division; Chondrocytes; Growth Plate; Mice; Telomere
BACKGROUND AND AIM: Telomeres are hexameric repeat sequences that flank eukaryotic chromosomes. The telomere hypothesis of cellular aging proposes that replication of normal somatic cells leads to progressive telomere shortening which induces replicative senescence. Previous studies suggest that growth plate chondrocytes have a finite proliferative capacity in vivo. We therefore hypothesized that telomere shortening in resting zone chondrocytes leads to replicative senescence.
METHOD: To test this hypothesis we compared the telomere restriction fragment (TRF) length of Mus casteneus at 1, 4, 8, and 56 weeks of age.
RESULTS AND CONCLUSIONS: We found that TRF length did not diminish measurably with age, suggesting that telomere shortening in resting zone chondrocytes is not the mechanism that limits proliferation of growth plate chondrocytes in vivo.
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Citation: Horm Res. 2005;63(3):125-8. Epub 2005 Mar 24. Link to article on publisher's site
Nwosu, Benjamin U.; Nilsson, Ola; Mitchum, Robert D. Jr.; Coco, Marilena; Barnes, Kevin M.; and Baron, Jeffrey, "Lack of telomere shortening with age in mouse resting zone chondrocytes" (2005). Pediatric Publications and Presentations. Paper 3.