Title

Characterization of serum anti-diphtheria antibody activity following administration of equine anti-toxin for suspected diphtheria

UMMS Affiliation

Department of Pediatrics, Division of Pediatric Immunology and Infectious Diseases; MassBiologics

Date

9-21-2017

Document Type

Article

Disciplines

Immunoprophylaxis and Therapy

Abstract

There is a global shortage of equine-derived diphtheria anti-toxin (DAT) for diphtheria treatment. There are few existing data on serum antibody concentrations and neutralizing activity post-treatment to support development of new therapeutics. Antibody concentrations were quantified by ELISA and anti-toxin neutralizing activity by cytotoxicity assay in serum from 4 patients receiving DAT for suspected diphtheria. Using linear mixed effects modeling, estimated mean (SE) half-life was 78.2 (20.0) hours. Maximum serum neutralizing activity ranged from 28.42-38.64 AU/mL with an estimated mean AUC1-72 of 1396.7 (399.3) AU/mL*hr. These data provide a standard of comparison for development of novel anti-toxins to replace DAT.

Rights and Permissions

Citation: Hum Vaccin Immunother. 2017 Sep 21:1-4. doi: 10.1080/21645515.2017.1362516. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

28933665