Title

Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1 Antitrypsin

UMMS Affiliation

Horae Gene Therapy Center; Department of Pediatrics, Division of Pulmonary and Allergy

Date

7-28-2017

Document Type

Book Chapter

Disciplines

Genetics and Genomics | Molecular Biology | Research Methods in Life Sciences

Abstract

This protocol describes the design, cloning, and in vitro screening of artificial microRNAs (miRNAs) to silence alpha-1 antitrypsin (AAT). This method would be of interest to silence AAT in a variety of in vitro or in vivo models, and prevalidated sequences against human AAT are provided. This simple 5-day protocol may more generally be used to design artificial miRNAs against any transcript.

Rights and Permissions

Citation: Methods Mol Biol. 2017;1639:107-114. doi: 10.1007/978-1-4939-7163-3_10. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Keywords

AAT, Alpha-1 antitrypsin, RNA interference, RNAi, Short hairpin RNA; Z-AAT, miRNA, microRNA, shRNA

PubMed ID

28752450