Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1 Antitrypsin
Horae Gene Therapy Center; Department of Pediatrics, Division of Pulmonary and Allergy
Genetics and Genomics | Molecular Biology | Research Methods in Life Sciences
This protocol describes the design, cloning, and in vitro screening of artificial microRNAs (miRNAs) to silence alpha-1 antitrypsin (AAT). This method would be of interest to silence AAT in a variety of in vitro or in vivo models, and prevalidated sequences against human AAT are provided. This simple 5-day protocol may more generally be used to design artificial miRNAs against any transcript.
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Citation: Methods Mol Biol. 2017;1639:107-114. doi: 10.1007/978-1-4939-7163-3_10. Link to article on publisher's site
AAT, Alpha-1 antitrypsin, RNA interference, RNAi, Short hairpin RNA; Z-AAT, miRNA, microRNA, shRNA
Borel, Florie and Mueller, Christian, "Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1 Antitrypsin" (2017). Pediatric Publications and Presentations. 163.