Lack of alternative coreceptor use by pediatric HIV-1 R5 isolates for infection of primary cord or adult peripheral blood mononuclear cells
Department of Pediatrics; Program in Molecular Medicine; Department of Medicine, Division of Diabetes; Department of Molecular Genetics and Microbiology
Medical Subject Headings
HIV Infections; HIV Reverse Transcriptase; HIV-1; Humans; Infant; Infant, Newborn; Leukocytes, Mononuclear; Receptors, CCR5; Receptors, Virus; *Virus Internalization; Virus Replication
Immunology and Infectious Disease | Pediatrics
HIV-1 infection of neonates results in an extended acute period of virus replication, frequent neurological problems and reduced survival compared to adults. In adults, R5 viruses mainly infect CCR5(+) CD4(+) memory T-cells. In neonates, CCR5(+) memory T-cells form a substantially smaller fraction of total lymphocytes. We therefore tested whether alternative coreceptors confer infection of lymphocytes by pediatric isolates. Pediatric HIV-1 R5 isolates failed to replicate in Delta32/Delta32 CCR5 PBMCs or in cord PBMCs treated with a CCR5 inhibitor. These results do not indicate a role for alternative coreceptors and provide support for CCR5 inhibitors in the therapy of HIV-1(+) neonates.
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Citation: Arch Virol. 2008;153(2):363-6. Epub 2007 Dec 13. Link to article on publisher's site