Title

Efficient replication of severe acute respiratory syndrome coronavirus in mouse cells is limited by murine angiotensin-converting enzyme 2

UMMS Affiliation

Department of Pediatrics; Program in Molecular Medicine

Date

10-2004

Document Type

Article

Medical Subject Headings

3T3 Cells; Animals; Cell Line; Endopeptidases; Humans; Mice; Peptidyl-Dipeptidase A; Rats; SARS Virus; Severe Acute Respiratory Syndrome; *Virus Replication

Disciplines

Immunology and Infectious Disease | Pediatrics

Abstract

Replication of viruses in species other than their natural hosts is frequently limited by entry and postentry barriers. The coronavirus that causes severe acute respiratory syndrome (SARS-CoV) utilizes the receptor angiotensin-converting enzyme 2 (ACE2) to infect cells. Here we compare human, mouse, and rat ACE2 molecules for their ability to serve as receptors for SARS-CoV. We found that, compared to human ACE2, murine ACE2 less efficiently bound the S1 domain of SARS-CoV and supported less-efficient S protein-mediated infection. Rat ACE2 was even less efficient, at near background levels for both activities. Murine 3T3 cells expressing human ACE2 supported SARS-CoV replication, whereas replication was less than 10% as efficient in the same cells expressing murine ACE2. These data imply that a mouse transgenically expressing human ACE2 may be a useful animal model of SARS.

Rights and Permissions

Citation: J Virol. 2004 Oct;78(20):11429-33. Link to article on publisher's site

Related Resources

Link to Article in PubMed