Title

Novel transcribed regions in the human genome

UMMS Affiliation

Department of Pediatrics

Date

12-2006

Document Type

Article

Medical Subject Headings

Cell Differentiation; Exons; Gene Expression Profiling; *Genome, Human; Humans; Introns; Neutrophils; Oligonucleotide Array Sequence Analysis; RNA; *Transcription, Genetic

Disciplines

Hematology | Oncology | Pediatrics

Abstract

We have used genomic tiling arrays to identify transcribed regions throughout the human genome. Analysis of the mapping results of RNA isolated from five cell/tissue types, NB4 cells, NB4 cells treated with retinoic acid (RA), NB4 cells treated with 12-O-tetradecanoylphorbol-13 acetate (TPA), neutrophils, and placenta, throughout the ENCODE region reveals a large number of novel transcribed regions. Interestingly, neutrophils exhibit a great deal of novel expression in several intronic regions. Comparison of the hybridization results of NB4 cells treated with different stimuli relative to untreated cells reveals that many new regions are expressed upon cell differentiation. One such region is the Hox locus, which contains a large number of novel regions expressed in a number of cell types. Analysis of the trinucleotide composition of the novel transcribed regions reveals that it is similar to that of known exons. These results suggest that many of the novel transcribed regions may have a functional role.

Rights and Permissions

Citation: Cold Spring Harb Symp Quant Biol. 2006;71:111-6. doi: 10.1101/sqb.2006.71.054

Related Resources

Link to article in PubMed

PubMed ID

17381286