Authors
Rozowsky, JoelWu, J.
Lian, Zheng
Nagalakshmi, U.
Korbel, J. O.
Kapranov, Philipp
Zheng, D.
Dyke, S.
Newburger, Peter E.
Miller, P.
Gingeras, Thomas R.
Weissman, Sherman M.
Gerstein, Mark B.
Snyder, Michael
UMass Chan Affiliations
Department of PediatricsDocument Type
Journal ArticlePublication Date
2006-12-01Keywords
Cell DifferentiationExons
Gene Expression Profiling
*Genome, Human
Humans
Introns
Neutrophils
Oligonucleotide Array Sequence Analysis
RNA
*Transcription, Genetic
Hematology
Oncology
Pediatrics
Metadata
Show full item recordAbstract
We have used genomic tiling arrays to identify transcribed regions throughout the human genome. Analysis of the mapping results of RNA isolated from five cell/tissue types, NB4 cells, NB4 cells treated with retinoic acid (RA), NB4 cells treated with 12-O-tetradecanoylphorbol-13 acetate (TPA), neutrophils, and placenta, throughout the ENCODE region reveals a large number of novel transcribed regions. Interestingly, neutrophils exhibit a great deal of novel expression in several intronic regions. Comparison of the hybridization results of NB4 cells treated with different stimuli relative to untreated cells reveals that many new regions are expressed upon cell differentiation. One such region is the Hox locus, which contains a large number of novel regions expressed in a number of cell types. Analysis of the trinucleotide composition of the novel transcribed regions reveals that it is similar to that of known exons. These results suggest that many of the novel transcribed regions may have a functional role.Source
Cold Spring Harb Symp Quant Biol. 2006;71:111-6. doi: 10.1101/sqb.2006.71.054DOI
10.1101/sqb.2006.71.054Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43410PubMed ID
17381286Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/sqb.2006.71.054