Title

A limited number of genes are involved in the differentiation of germinal center B cells

UMMS Affiliation

Department of Pediatrics

Date

12-1-2006

Document Type

Article

Medical Subject Headings

Animals; B-Lymphocytes; Cell Differentiation; Cell Line; Child; Child, Preschool; DNA-Binding Proteins; Dithiothreitol; Gene Expression Profiling; Germinal Center; Humans; Molecular Sequence Data; Nuclear Proteins; Oligonucleotide Array Sequence Analysis; Palatine Tonsil; Protein Folding; Transcription Factors; Tunicamycin

Disciplines

Hematology | Oncology | Pediatrics

Abstract

Mature B cells, upon activation, progressively differentiate through centroblasts into centrocytes and finally to plasmacytes that express large amounts of selected immunoglobulins. A significant part of this maturation is thought to involve induction of the unfolded protein response (UPR). We have compared gene expression in normal germinal center centroblasts, centrocytes, lymphoblastoid cells undergoing induced UPR, and the CCL155 plasmacytoma cell line. In the centroblast to centrocyte transition there is a change in the expression of a relatively small number of genes. These include a limited subset of the genes upregulated by a fully activated UPR as well as a small number of other transcription factors, some disulphide isomerases, and other genes. This is consistent with a model in which this transition is mediated by changes in the levels of expression of transcription factor B-lymphocyte-induced maturation protein 1 (Blimp1) (PRDM1), BACH2, X-box binding protein 1 (XBP1), interferon regulatory factor 4 (IRF4), and possibly vitamin D receptor (VDR) expression, together with post-transcriptional changes and a limited induction of aspects of the UPR.

Rights and Permissions

Citation: J Cell Biochem. 2006 Dec 1;99(5):1308-25. doi: 10.1002/jcb.20952

Related Resources

Link to article in PubMed

PubMed ID

16795035