Title

Elevated plasma glycocalicin levels and decreased ristocetin-induced platelet agglutination in hemodialysis patients

UMMS Affiliation

Department of Pediatrics

Date

7-1998

Document Type

Article

Medical Subject Headings

Blood Platelets; Case-Control Studies; Humans; Peritoneal Dialysis; Platelet Aggregation; Platelet Aggregation Inhibitors; Platelet Glycoprotein GPIb-IX Complex; *Renal Dialysis; Ristocetin; Uremia

Disciplines

Hematology | Oncology | Pediatrics

Abstract

A bleeding diathesis caused by platelet dysfunction is a major cause of morbidity and mortality in patients with uremia. Platelet adhesion to vascular subendothelium is defective in uremia and depends on the interactions of the platelet glycoprotein (GP) Ib/IX complex with the vascular wall. We measured levels of platelet surface GPIb, platelet surface GPIX, plasma glycocalicin (a product of enzymatic cleavage of GPIb), and ristocetin-induced platelet agglutination (RIPA) in patients undergoing chronic hemodialysis compared with patients undergoing peritoneal dialysis and healthy controls. Patients undergoing chronic maintenance hemodialysis have higher levels of platelet surface expression of GPIb (187+/-10 fluorescent units; P andlt; 0.001) than either healthy controls (120+/-4 fluorescent units; P andlt; 0.001) or patients undergoing peritoneal dialysis (127+/-5 fluorescent units; P andlt; 0.001). Similar changes were observed in platelet surface GPIX. Plasma glycocalicin levels were elevated in chronic hemodialysis patients (71+/-5 nmol/L) compared with healthy controls (36+/-3 nmol/L; P andlt; 0.001). Plasma glycocalicin levels also increased progressively throughout the hemodialysis procedure. The slope of RIPA was significantly lower in chronic hemodialysis patients (46+/-3) than in either healthy controls (67+/-4; P andlt; 0.05) or peritoneal dialysis patients (62+/-2; P andlt; 0.05). In conclusion, patients undergoing chronic maintenance hemodialysis have increased plasma glycocalicin levels and decreased RIPA, which may contribute to diminished platelet adhesion to vascular subendothelium and increased bleeding associated with uremia.

Rights and Permissions

Citation: Am J Kidney Dis. 1998 Jul;32(1):132-8. doi 10.1053/ajkd.1998.v32.pm9669434

Related Resources

Link to article in PubMed

PubMed ID

9669434