Authors
Linden, Matthew DeanFrelinger, Andrew L. III
Barnard, Marc R.
Przyklenk, Karin
Furman, Mark I.
Michelson, Alan D.
UMass Chan Affiliations
Department of AnesthesiologyDepartment of Emergency Medicine
Department of Pediatrics
Document Type
Journal ArticlePublication Date
2004-10-01Keywords
Blood Platelet DisordersBlood Platelets
Coronary Disease
Flow Cytometry
Humans
Phenotype
Platelet Activation
Syndrome
Thrombocytopenia
Hematology
Oncology
Pediatrics
Metadata
Show full item recordAbstract
Flow cytometry is a powerful and versatile tool that can be used to yield definitive information regarding the phenotypic status of platelets. The method provides a quantitative assessment of the physical and antigenic properties of platelets (e.g., surface expression of receptors, bound ligands, components of granules, or interactions of platelets with other platelets, other blood cells, or components of the plasma coagulation system), thereby facilitating the diagnosis of inherited or acquired platelet disorders (e.g., Bernard-Soulier syndrome, Glanzmann thrombasthenia, storage pool disease), the pathological activation of platelets (e.g., in the setting of acute coronary syndromes, cerebrovascular ischemia, peripheral vascular disease, cardiopulmonary bypass), and changes in the ability of platelets to activate via specific stimuli (e.g., efficacy of antiplatelet therapies). Accordingly, this review summarizes the key technical and methodologic components of flow cytometric analysis of platelets, as well as specific examples of its application to diagnosis and patient care.Source
Semin Thromb Hemost. 2004 Oct;30(5):501-11. doi 10.1055/s-2004-835671DOI
10.1055/s-2004-835671Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43381PubMed ID
15497093Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1055/s-2004-835671