Title

Effect of perineal cleansing on contamination rate of mid-stream urine culture

UMMS Affiliation

Department of Pediatrics

Date

2-14-2006

Document Type

Article

Medical Subject Headings

Adolescent; Adult; Antisepsis; Bacteriuria; Colony Count, Microbial; Female; Humans; Perineum; Specimen Handling; Urinary Tract Infections; Urine

Disciplines

Pediatrics

Abstract

STUDY OBJECTIVE: Urinary tract infection (UTI) and chlamydial or gonococcal urethritis are the most common causes of female dysuria. While chlamydia and gonorrhea can be detected with a nucleic acid amplification test performed on an uncleansed first part voided urine sample, urine cultures to test for UTI require a mid-stream clean caught sample. In order to determine whether collecting two sequential non-clean caught urine samples during the same void to test for chlamydia, gonorrhea, and UTI is a reasonable approach, we assessed the degree to which perineal cleansing reduces bacterial contamination of mid-stream urine cultures. DESIGN: Experimental study comparing mid-stream urine samples collected with (n = 25) and without (n = 25) perineal cleansing.

SETTING: A university-based adolescent clinic

PARTICIPANTS: We recruited fifty 14-23-year-old (mean 18.5 yrs, SD 2.3) asymptomatic females.

MAIN OUTCOME MEASURE: Perineal flora contamination rate of mid-stream urine cultures collected with and without perineal cleansing.

RESULTS: No culture grew >10(4) colonies of a pathogenic bacterium. Eleven (44%) of the experimental group samples and 9 (36%) of the control samples grew >10(4) colonies of perineal bacterial flora (chi(2) = .33, P = 0.56). Participants' previous experience collecting mid-stream urine was not associated with less bacterial contamination.

CONCLUSIONS: Among this small sample of asymptomatic young women, perineal cleansing did not significantly reduce perineal flora contamination of mid-stream urine cultures. If larger studies of symptomatic young women replicate these findings, young women could collect two sequential urine samples from the same void to test for chlamydia, gonorrhea, and UTI.

Rights and Permissions

Citation: J Pediatr Adolesc Gynecol. 2006 Feb;19(1):31-4. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

16472726