Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry
Department of Orthopedics and Physical Rehabilitation
Medical Subject Headings
Alkynes; Animals; Azides; Biocompatible Materials; Bone Marrow Cells; Cell Survival; *Click Chemistry; Cyclization; Heterocyclic Compounds, 3-Ring; Hydrogels; Male; Molecular Structure; Polyethylene Glycols; Rats
Biochemistry, Biophysics, and Structural Biology | Cell and Developmental Biology | Medicine and Health Sciences
Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by "clicking" azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells encapsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.
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Citation: 24. Link to article on publisher's site
Xu, Jianwen; Filion, Tera M.; Prifti, Fioleda; and Song, Jie, "Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry" (2011). Orthopedics and Physical Rehabilitation Publications and Presentations. 172.