Title

Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry

UMMS Affiliation

Department of Orthopedics and Physical Rehabilitation

Date

10-2011

Document Type

Article

Medical Subject Headings

Alkynes; Animals; Azides; Biocompatible Materials; Bone Marrow Cells; Cell Survival; *Click Chemistry; Cyclization; Heterocyclic Compounds, 3-Ring; Hydrogels; Male; Molecular Structure; Polyethylene Glycols; Rats

Disciplines

Biochemistry, Biophysics, and Structural Biology | Cell and Developmental Biology | Medicine and Health Sciences

Abstract

Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by "clicking" azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells encapsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.

Rights and Permissions

Citation: 24. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

21954076