In vivo tissue responses to thermal-responsive shape memory polymer nanocomposites
Department of Cell Biology; Department of Orthopedics and Physical Rehabilitation
Medical Subject Headings
Animals; Biocompatible Materials; Foreign-Body Reaction; Implants, Experimental; Male; Materials Testing; Molecular Structure; Nanocomposites; Polymers; Rats; Rats, Sprague-Dawley; *Temperature; Tissue Engineering; Tissue Scaffolds
Orthopedics | Rehabilitation and Therapy
To explore the safe use of thermal-responsive shape memory polymers (SMPs) as minimally invasive tissue scaffolds, we recently developed a class of biodegradable POSS-SMP nanocomposites exhibiting stable temporary shape fixing and facile shape recovery within a narrow window of physiological temperatures. The materials were covalently crosslinked from star-branched building blocks consisting a bioinert polyhedral oligomeric silsesquioxane (POSS) core and 8 degradable poly(D,L-lactide) (PLA) arms. Here we examine the degradation profiles and immunogenicity of POSS-SMPs as a function of the PLA arm lengths using a rat subcutaneous implantation model. We show that POSS-SMPs elicited a mild foreign body type immune response upon implantation. The degradation rates of POSS-SMPs, both in vitro and in vivo, inversely correlated with the length of the PLA chains within the crosslinked amorphous network. Upon in vivo degradation of POSS-SMPs, a second acute inflammatory response was elicited locally, and the inflammation was able to resolve over time without medical interventions. One year after the implantation of POSS-SMPs, no pathologic abnormalities were detected from the vital/scavenger organs examined. These minimally immunogenic and biodegradable SMPs are promising candidates for scaffold-assisted tissue repair where both facile surgical delivery and controlled degradation of the scaffold are desired for achieving optimal short-term and long-term clinical outcomes.
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Citation: Biomaterials. 2011 Feb;32(4):985-91. Epub 2010 Oct 30. Link to article on publisher's site