Title

Recombinant human thyrotropin stimulates thyroid function and radioactive iodine uptake in the rhesus monkey

UMMS Affiliation

Division of Endocrinology

Date

5-1-1992

Document Type

Article

Subjects

Animals; Female; Humans; Iodine Radioisotopes; Macaca mulatta; Metabolic Clearance Rate; Recombinant Proteins; Thyroid Gland; Thyroid Hormones; Thyrotropin

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The administration of bovine TSH to stimulate thyroid radioactive iodine uptake to detect functioning thyroid tissue in man after surgery for thyroid cancer is rarely, if ever, used, due to allergic reactions and/or the development of TSH antibodies. Human (h) TSH would be far less likely to induce allergic reactions or TSH antibodies. Recombinant hTSH (rec-hTSH) was produced by a line of Chinese hamster ovary cells that had been transfected with cDNA for the two subunit proteins that comprise hTSH. The present study was carried out to determine the half-life of rec-hTSH in the monkey and its ability to stimulate thyroid function. The half-life of rec-hTSH after iv administration was approximately 63 min for the rapid phase and 326 min for the slow phase. After three daily im injections of 2 U rec-hTSH to two monkeys, serum T4 concentrations increased several-fold, and serum T3 increased 2-3 times above basal values. The 6 and 20 h thyroid 123I uptakes doubled after rec-hTSH administration. These results demonstrate the biological efficacy of rec-hTSH administered to the monkey and strongly suggest that rec-hTSH will be effective in stimulating thyroid function in man.

Rights and Permissions

Citation: J Clin Endocrinol Metab. 1992 May;74(5):1135-9.

Related Resources

Link to Article in PubMed

Journal Title

The Journal of clinical endocrinology and metabolism

PubMed ID

1569160