A progesterone-induced endometrial homolog of a new candidate tumor suppressor, DMBT1
Department of Obstetrics and Gynecology; Department of Physiology
*Agglutinins; Amino Acid Sequence; Animals; Endometrium; Female; Gene Expression; Genes, Tumor Suppressor; Humans; In Situ Hybridization; Macaca mulatta; Molecular Sequence Data; Polymerase Chain Reaction; Progesterone; Receptors, Cell Surface; Sequence Homology, Amino Acid
Life Sciences | Medicine and Health Sciences
We have previously prepared and characterized a subtracted library enriched for endometrial progesterone (P)-dependent genes in the rhesus monkey. One of the fragment clones (H3) that we selected for sequencing from this library was found to be homologous to human DMBT1, a recently isolated member of the scavenger receptor cysteine-rich superfamily and a new putative tumor suppressor. In this report, we provide evidence that H3 is the rhesus monkey homolog of DMBT1. Additional sequence data of H3 (1071 bp) showed a striking homology with DMBT1 (92% identical). Semiquantitative kinetic PCR of estrogen-dominant vs. P-dominant endometrial complementary DNA populations showed that the H3 gene was up-regulated 5-fold by normal secretory P levels. In situ hybridization with unique probes to H3 confirmed the up-regulation by P in the endometrium and a restricted expression in the stromal compartment. Another recent report suggested the presence of an endometrial tumor suppressor in the same chromosomal region as DMBT1 (10q23-26); deletions in this region were associated with endometrial cancers. Together, these studies potentially provide a molecular link to the protective effect of the action of P on unopposed estrogen exposure in reproductive tract cancers in women.
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Citation: J Clin Endocrinol Metab. 1998 Oct;83(10):3569-73.
The Journal of clinical endocrinology and metabolism
Ace, Christopher I. and Okulicz, William C., "A progesterone-induced endometrial homolog of a new candidate tumor suppressor, DMBT1" (1998). Open Access Articles. 984.