Anti-CD2 monoclonal antibodies prevent spontaneous and adoptive transfer of diabetes in the BB/Wor rat
Department of Pathology
Animals; Antibodies, Monoclonal; Antigens, CD2; Antigens, CD4; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Autoimmune Diseases; Concanavalin A; Diabetes Mellitus, Type 1; Flow Cytometry; Hyperinsulinism; *Immunotherapy, Adoptive; Injections; Lymph Nodes; Poly I-C; Rats; Rats, Inbred BB; Receptors, Immunologic; Spleen; T-Lymphocytes
Life Sciences | Medicine and Health Sciences
We studied the effects of anti-CD2 monoclonal antibodies (MAb) on spontaneous and induced autoimmune diabetes mellitus in diabetes-prone (DP) and diabetes-resistant (DR) BB/Wor rats. In DP rats, all anti-CD2 MAb prevented spontaneous diabetes and the adoptive transfer of diabetes with Con-A--stimulated acute diabetic spleen cells; OX34 prevented Poly I:C induced accelerated onset of diabetes and the adoptive transfer of diabetes with Con-A--stimulated RT6.1+ T cell depleted DR splenocytes. In DP rats, all anti-CD2 MAb except OX53 depleted CD4+ T cells, without depleting natural killer cells or CD8+ T cells. OX34 injected DR rats were profoundly depleted of CD4+ T cells without evidence of decreased CD8+ T cells, but were not protected against the induction of diabetes by RT6.1+ T-cell depletion and Poly I:C injections. We conclude that anti-CD2 MAbs protect against BB/Wor autoimmune diabetes by depleting CD4+ T cells, preventing the activation of effector cells, or by blocking CD2/ligand interaction between effector and target cells.
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Citation: Am J Pathol. 1992 Nov;141(5):1043-51.
The American journal of pathology
Barlow, Avlin K. and Like, Arthur A., "Anti-CD2 monoclonal antibodies prevent spontaneous and adoptive transfer of diabetes in the BB/Wor rat" (1992). Open Access Articles. 97.