Title

Anti-CD2 monoclonal antibodies prevent spontaneous and adoptive transfer of diabetes in the BB/Wor rat

UMMS Affiliation

Department of Pathology

Publication Date

11-1-1992

Document Type

Article

Subjects

Animals; Antibodies, Monoclonal; Antigens, CD2; Antigens, CD4; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Autoimmune Diseases; Concanavalin A; Diabetes Mellitus, Type 1; Flow Cytometry; Hyperinsulinism; *Immunotherapy, Adoptive; Injections; Lymph Nodes; Poly I-C; Rats; Rats, Inbred BB; Receptors, Immunologic; Spleen; T-Lymphocytes

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We studied the effects of anti-CD2 monoclonal antibodies (MAb) on spontaneous and induced autoimmune diabetes mellitus in diabetes-prone (DP) and diabetes-resistant (DR) BB/Wor rats. In DP rats, all anti-CD2 MAb prevented spontaneous diabetes and the adoptive transfer of diabetes with Con-A--stimulated acute diabetic spleen cells; OX34 prevented Poly I:C induced accelerated onset of diabetes and the adoptive transfer of diabetes with Con-A--stimulated RT6.1+ T cell depleted DR splenocytes. In DP rats, all anti-CD2 MAb except OX53 depleted CD4+ T cells, without depleting natural killer cells or CD8+ T cells. OX34 injected DR rats were profoundly depleted of CD4+ T cells without evidence of decreased CD8+ T cells, but were not protected against the induction of diabetes by RT6.1+ T-cell depletion and Poly I:C injections. We conclude that anti-CD2 MAbs protect against BB/Wor autoimmune diabetes by depleting CD4+ T cells, preventing the activation of effector cells, or by blocking CD2/ligand interaction between effector and target cells.

Rights and Permissions

Citation: Am J Pathol. 1992 Nov;141(5):1043-51.

Related Resources

Link to article in PubMed

Journal/Book/Conference Title

The American journal of pathology

PubMed ID

1359788