UMMS Affiliation

Department of Cell Biology

Publication Date

12-1-1990

Document Type

Article

Subjects

Actins; Animals; Cells, Cultured; Chick Embryo; Cycloheximide; DNA Probes; Emetine; Fibroblasts; Kinetics; Muscles; Nucleic Acid Hybridization; Protein Biosynthesis; Protein Synthesis Inhibitors; Puromycin; RNA Processing, Post-Transcriptional; RNA, Messenger; Sodium Fluoride

Disciplines

Cell Biology

Abstract

Actin mRNA is localized in chicken embryo fibroblasts to the distal regions of leading lamellae, but not within the ruffling edges. In this investigation we have addressed the role of actin translation in this process. The translocation of actin mRNA to the cell periphery was studied by monitoring the distribution of actin mRNA in cells during spreading. Within 90 min, actin mRNA moved from a perinuclear to a peripheral distribution. Formation of lamellipodia preceded actin mRNA localization, indicating that localization is not a prerequisite for this event. Neither puromycin (which dissociates ribosomes from mRNA) nor cycloheximide (which stabilizes ribosomes on mRNA) had any effect on this movement of actin mRNA. Anchoring of actin mRNA was studied using cells with peripherally localized actin mRNA. No change in actin mRNA localization was observed for 30 min in the same inhibitors. These data indicate that the presence of the nascent polypeptide is not necessary for translocation of actin mRNA to the cell periphery, or anchoring at that site. This suggests that the mRNA contains information concerning its spatial distribution within the cytoplasm.

Rights and Permissions

Citation: J Cell Biol. 1990 Dec;111(6 Pt 1):2397-403.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The Journal of cell biology

PubMed ID

2277064

Included in

Cell Biology Commons

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