Title

Polyinosinic:polycytidylic acid is a potent activator of endothelial cells

UMMS Affiliation

Department of Medicine; Department of Pathology

Date

7-1-1994

Document Type

Article

Subjects

Animals; Cell Adhesion Molecules; Cells, Cultured; Cytokines; Endothelium, Vascular; Humans; Interferons; Interleukins; Pancreas; Pancreatitis; Poly I-C; Rats; Rats, Inbred BB; Rats, Inbred WF

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Polyinosinic:polycytidylic acid (poly I:C) is a synthetic double-stranded polyribonucleotide that elicits immune responses analogous to those observed during viral infection. It is also known to modulate the expression of certain autoimmune disorders including diabetes mellitus in the BB rat and NOD mouse. The mechanism underlying these immunomodulatory effects is not known, but it could involve activation of vascular endothelium. We now report that parenteral poly I:C induces rat pancreatic endothelium to hyperexpress intercellular adhesion molecule 1 (CD54). This is accompanied by a perivascular recruitment of mononuclear cells to the exocrine pancreas. Corollary in vitro studies demonstrated that poly I:C is a potent activator of both rat and human endothelial cells in culture. It upregulates endothelial expression of several leukocyte adhesion molecules, stimulates the release of interleukin-6 and interleukin-8, and antagonizes interferon-gamma induction of major histocompatibility complex class II expression. We conclude that poly I:C activates endothelial cells to express surface molecules and cytokines in a pattern classically associated with leukocyte recruitment. These effects may in part contribute to the immunomodulatory effects of poly I:C in animal models of autoimmunity.

Rights and Permissions

Citation: Am J Pathol. 1994 Jul;145(1):137-47.

Related Resources

Link to article in PubMed

Journal Title

The American journal of pathology

PubMed ID

7518192