Title

Reversible endothelial cell relaxation induced by oxygen and glucose deprivation. A model of ischemia in vitro

UMMS Affiliation

Department of Pathology

Publication Date

7-1-1994

Document Type

Article

Subjects

Animals; Cell Count; Cell Hypoxia; Cells, Cultured; Endothelium, Vascular; Glucose; Ischemia; Microfilament Proteins; Models, Biological; Muscle Contraction; Muscle Relaxation; Muscle, Smooth, Vascular; Rats; Time Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Endothelial cells (EC) cultured on polymerized silicone deform the underlying substrate, producing microscopically visible wrinkles. This has been interpreted as cellular contraction, and we have previously concluded that EC normally maintain an active contractile tone. Since in ischemic tissues capillaries become "paralyzed" and lose their tone, we decided to examine the effects of glucose and/or oxygen deprivation on EC contractility. Contracting cultures with wrinkled silicone substrates were exposed to complete anoxia with or without exogenous glucose and followed by time-lapse photography. Under either glucose-or oxygen-free conditions, contraction was maintained for up to 4 days. If, however, both oxygen and glucose were removed, cellular contraction was reversed. After a period of 2 to 4 hours substrate wrinkles gradually disappeared, until by 3 to 7 hours, few to no wrinkles remained. Furthermore, within 10 minutes of restoration to normal oxygen (but not glucose) levels, substrate wrinkling reappeared. F-actin microfilament patterns and cell number per unit area were also altered by glucose and oxygen deprivation. Similar results were obtained using large or small vessel EC. We conclude that in the absence of glucose and oxygen EC lose their contractile tone, and that tone can be re-established upon re-exposure to oxygen. These findings should have implications for the pathogenesis of capillary paralysis in ischemia.

Rights and Permissions

Citation: Am J Pathol. 1994 Jul;145(1):211-9.

Related Resources

Link to article in PubMed

Journal/Book/Conference Title

The American journal of pathology

PubMed ID

8030750