UMMS Affiliation

Program in Molecular Medicine; Department of Cell Biology

Publication Date

12-29-1998

Document Type

Article

Subjects

Animals; Biological Transport; COS Cells; Cell Line; Cell Nucleus; Cricetinae; Hela Cells; Humans; Immunohistochemistry; Kidney; Macromolecular Substances; Mesocricetus; Microscopy, Fluorescence; Microscopy, Immunoelectron; Models, Biological; Nuclear Envelope; Nuclear Pore Complex Proteins; Nuclear Proteins; Protein Binding; *Protein Structure, Tertiary; Proteins; Proto-Oncogene Proteins; RNA, Messenger; Sequence Deletion; Structure-Activity Relationship; Transfection

Disciplines

Cell Biology

Abstract

Tpr is a 270-kD coiled-coil protein localized to intranuclear filaments of the nuclear pore complex (NPC). The mechanism by which Tpr contributes to the structure and function of the nuclear pore is currently unknown. To gain insight into Tpr function, we expressed the full-length protein and several subdomains in mammalian cell lines and examined their effects on nuclear pore function. Through this analysis, we identified an NH2-terminal domain that was sufficient for association with the nucleoplasmic aspect of the NPC. In addition, we unexpectedly found that the acidic COOH terminus was efficiently transported into the nuclear interior, an event that was apparently mediated by a putative nuclear localization sequence. Ectopic expression of the full-length Tpr caused a dramatic accumulation of poly(A)+ RNA within the nucleus. Similar results were observed with domains that localized to the NPC and the nuclear interior. In contrast, expression of these proteins did not appear to affect nuclear import. These data are consistent with a model in which Tpr is tethered to intranuclear filaments of the NPC by its coiled coil domain leaving the acidic COOH terminus free to interact with soluble transport factors and mediate export of macromolecules from the nucleus.

Rights and Permissions

Citation: J Cell Biol. 1998 Dec 28;143(7):1801-12.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The Journal of cell biology

PubMed ID

9864356

Included in

Cell Biology Commons

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