Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis
UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyDocument Type
Journal ArticlePublication Date
1999-08-25Keywords
Actins*Cell Cycle Proteins
*Cell Division
Cytoskeleton
*DNA-Binding Proteins
Epistasis, Genetic
Fungal Proteins
Genes, Fungal
Genes, cdc
Microtubules
Mitotic Spindle Apparatus
Mutation
Protein Kinases
Recombinant Fusion Proteins
*Saccharomyces cerevisiae Proteins
Schizosaccharomyces
*Schizosaccharomyces pombe Proteins
Signal Transduction
Temperature
Time Factors
Microbiology
Molecular Genetics
Metadata
Show full item recordAbstract
The fission yeast Schizosaccharomyces pombe divides by medial fission through the use of an actomyosin contractile ring. Precisely at the end of anaphase, the ring begins to constrict and the septum forms. Proper coordination of cell division with mitosis is crucial to ensure proper segregation of chromosomes to daughter cells. The Sid2p kinase is one of several proteins that function as part of a novel signaling pathway required for initiation of medial ring constriction and septation. Here, we show that Sid2p is a component of the spindle pole body at all stages of the cell cycle and localizes transiently to the cell division site during medial ring constriction and septation. A medial ring and an intact microtubule cytoskeleton are required for the localization of Sid2p to the division site. We have established an in vitro assay for measuring Sid2p kinase activity, and found that Sid2p kinase activity peaks during medial ring constriction and septation. Both Sid2p localization to the division site and activity depend on the function of all of the other septation initiation genes: cdc7, cdc11, cdc14, sid1, spg1, and sid4. Thus, Sid2p, a component of the spindle pole body, by virtue of its transient localization to the division site, appears to determine the timing of ring constriction and septum delivery in response to activating signals from other Sid gene products.Source
J Cell Biol. 1999 Aug 23;146(4):777-90.Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42600PubMed ID
10459013Related Resources
Link to Article in PubMedCollections
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