UMMS Affiliation

Department of Cell Biology

Publication Date

9-17-2003

Document Type

Article

Subjects

Cell Line; Cell Nucleus; Chromosome Banding; Chromosome Structures; Collagen Type I; DNA Replication; Euchromatin; Eukaryotic Cells; Fluorescent Antibody Technique; Gene Expression Regulation; Humans; Multigene Family; Nuclear Proteins; RNA, Messenger; *Ribonucleoproteins

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

Typically, eukaryotic nuclei contain 10-30 prominent domains (referred to here as SC-35 domains) that are concentrated in mRNA metabolic factors. Here, we show that multiple specific genes cluster around a common SC-35 domain, which contains multiple mRNAs. Nonsyntenic genes are capable of associating with a common domain, but domain "choice" appears random, even for two coordinately expressed genes. Active genes widely separated on different chromosome arms associate with the same domain frequently, assorting randomly into the 3-4 subregions of the chromosome periphery that contact a domain. Most importantly, visualization of six individual chromosome bands showed that large genomic segments ( approximately 5 Mb) have striking differences in organization relative to domains. Certain bands showed extensive contact, often aligning with or encircling an SC-35 domain, whereas others did not. All three gene-rich reverse bands showed this more than the gene-poor Giemsa dark bands, and morphometric analyses demonstrated statistically significant differences. Similarly, late-replicating DNA generally avoids SC-35 domains. These findings suggest a functional rationale for gene clustering in chromosomal bands, which relates to nuclear clustering of genes with SC-35 domains. Rather than random reservoirs of splicing factors, or factors accumulated on an individual highly active gene, we propose a model of SC-35 domains as functional centers for a multitude of clustered genes, forming local euchromatic "neighborhoods."

Rights and Permissions

Citation: J Cell Biol. 2003 Sep 15;162(6):981-90. Link to article on publisher's site

DOI of Published Version

10.1083/jcb.200303131

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The Journal of cell biology

PubMed ID

12975345

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