UMMS Affiliation

Department of Cell Biology

Date

6-9-2004

Document Type

Article

Subjects

Animals; Cell Line, Transformed; Cell Nucleus; Cytochalasin D; DNA; Fibroblasts; G1 Phase; Genes, cdc; Heterocyclic Compounds with 4 or More Rings; Humans; Mitosis; *Polyploidy; Rats

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

Failure of cells to cleave at the end of mitosis is dangerous to the organism because it immediately produces tetraploidy and centrosome amplification, which is thought to produce genetic imbalances. Using normal human and rat cells, we reexamined the basis for the attractive and increasingly accepted proposal that normal mammalian cells have a "tetraploidy checkpoint" that arrests binucleate cells in G1, thereby preventing their propagation. Using 10 microM cytochalasin to block cleavage, we confirm that most binucleate cells arrest in G1. However, when we use lower concentrations of cytochalasin, we find that binucleate cells undergo DNA synthesis and later proceed through mitosis in >80% of the cases for the hTERT-RPE1 human cell line, primary human fibroblasts, and the REF52 cell line. These observations provide a functional demonstration that the tetraploidy checkpoint does not exist in normal mammalian somatic cells.

Rights and Permissions

Citation: J Cell Biol. 2004 Jun 7;165(5):609-15. Link to article on publisher's site

DOI of Published Version

10.1083/jcb.200403014

Related Resources

Link to Article in PubMed

Journal Title

The Journal of cell biology

PubMed ID

15184397

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