Reconstitution of epidermal growth factor receptor transmodulation by platelet-derived growth factor in Chinese hamster ovary cells
Department of Biochemistry; Program in Molecular Medicine
Animals; Cell Line; Cricetinae; Cricetulus; DNA; DNA Replication; Female; Humans; Kinetics; Ovary; Platelet-Derived Growth Factor; Protein Kinase C; Receptor, Epidermal Growth Factor; Receptors, Cell Surface; Receptors, Platelet-Derived Growth Factor; Tetradecanoylphorbol Acetate; Transfection
Life Sciences | Medicine and Health Sciences
Platelet-derived growth factor (PDGF) causes an acute decrease in the high affinity binding of epidermal growth factor (EGF) to cell surface receptors and an increase in the phosphorylation state of the EGF receptor at threonine654. The hypothesis that PDGF action to regulate the EGF receptor is mediated by the activation of protein kinase C and the subsequent phosphorylation of EGF receptor threonine654 was tested. The human receptors for PDGF and EGF were expressed in Chinese hamster ovary cells that lack expression of endogenous receptors for these growth factors. The heterologous regulation of the EGF receptor by PDGF was reconstituted in cells expressing [Thr654]EGF receptors or [Ala654]EGF receptors. PDGF action was also observed in phorbol ester down-regulated cells that lack detectable protein kinase C activity. Together these data indicate that neither protein kinase C nor the phosphorylation of EGF receptor threonine654 is required for the regulation of the apparent affinity of the EGF receptor by PDGF.
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Citation: J Biol Chem. 1989 Aug 15;264(23):13642-7.
The Journal of biological chemistry
Countaway, Janice L.; Girones, Nuria; and Davis, Roger J., "Reconstitution of epidermal growth factor receptor transmodulation by platelet-derived growth factor in Chinese hamster ovary cells" (1989). Open Access Articles. 877.