Title

Disassembly of Son-of-sevenless proteins from Grb2 during p21ras desensitization by insulin

UMMS Affiliation

Program in Molecular Medicine

Publication Date

1-27-1995

Document Type

Article

Subjects

3T3 Cells; *Adaptor Proteins, Signal Transducing; Adipocytes; Animals; Antibodies; Electrophoresis, Polyacrylamide Gel; GRB2 Adaptor Protein; Guanosine Triphosphate; Immunoblotting; Kinetics; Membrane Proteins; Mice; Phosphorus Radioisotopes; Phosphorylation; Protein Binding; Proteins; Proto-Oncogene Proteins p21(ras); Receptor, Epidermal Growth Factor; Receptor, Insulin; Signal Transduction; Son of Sevenless Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Insulin receptor signaling acutely stimulates GTP loading of p21ras, apparently by mobilizing complexes of Grb2 and the guanine nucleotide exchangers Son-of-sevenless (Sos) 1 and 2 to associate with tyrosine-phosphorylated proteins in the plasma membrane. Here we show that in 32P-labeled 3T3-L1 adipocytes the elevated cellular concentrations of [32P]GTP-bound p21ras in response to insulin return to near basal levels after 20-30 min of hormone stimulation, while insulin receptors remain activated. Lysates of such desensitized cells were quantitatively immunoprecipitated with an antiserum recognizing both Sos1 and Sos2 proteins or a specific anti-Sos2 antiserum. Immunoblot analysis of these precipitates revealed that insulin causes a marked hyperphosphorylation of Sos1 and a 50% decrease in Grb2 associated with Sos proteins under these conditions. Similarly, anti-Grb2 immunoprecipitates of such lysates revealed the presence of decreased Sos1 protein due to insulin action. The disassembly of Grb2 from Sos proteins slightly precedes the time course of p21ras deactivation in response to insulin. These data are consistent with the hypothesis that the dissociation of Grb2 from Sos proteins caused by insulin in 3T3-L1 cells mediates p21ras deactivation and desensitization.

Rights and Permissions

Citation: J Biol Chem. 1995 Jan 27;270(4):1485-8.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The Journal of biological chemistry

PubMed ID

7829473