Determination of human myosin III as a motor protein having a protein kinase activity
Department of Physiology
Actins; Adenosine Triphosphatases; Chromatography, Gel; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Humans; Immunoblotting; Kinetics; Myosin Type III; Phosphorylation; Protein Binding; Protein Kinases; Protein Structure, Tertiary; Protein Transport; Recombinant Proteins; Time Factors
Life Sciences | Medicine and Health Sciences
The class III myosin is the most divergent member of the myosin superfamily, having a domain with homology to a protein kinase. However, the function of class III myosin at a molecular level is not known at all, and it has been questioned whether it is actually an actin-based motor molecule. Here, we showed that human myosin III has an ATPase activity that is significantly activated by actin (20-fold) with Kactin of 112 microm and Vmax of 0.34 s-1, indicating the mechanoenzymatic activity of myosin III. Furthermore, we found that human myosin III has actin translocating activity (0.11 +/- 0.05 microm/s) using an in vitro actin gliding assay, and it moves toward the plus end of actin filaments. Myosin III containing calmodulin as the light chain subunit showed a protein kinase activity and underwent autophosphorylation. The autophosphorylation was the intramolecular process, and the sites were at the C-terminal end of the motor domain. Autophosphorylation significantly activated the kinase activity, although it did not affect the ATPase activity. The present study is the first report that clearly demonstrates that the class III myosin is an actin-based motor protein having a protein kinase activity.
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Citation: J Biol Chem. 2003 Jun 13;278(24):21352-60. Epub 2003 Apr 2. Link to article on publisher's site
DOI of Published Version
The Journal of biological chemistry
Komaba, Shigeru; Inoue, Akira; Maruta, Shinsaku; Hosoya, Hiroshi; and Ikebe, Mitsuo, "Determination of human myosin III as a motor protein having a protein kinase activity" (2003). Open Access Articles. 719.