Title

Deficiency of the zinc finger protein ZPR1 causes defects in transcription and cell cycle progression

UMMS Affiliation

Program in Molecular Medicine

Publication Date

10-28-2006

Document Type

Article

Subjects

Carrier Proteins; Cell Cycle; Cell Cycle Proteins; Cell Line; Cell Survival; DNA Replication; G1 Phase; G2 Phase; Hela Cells; Histones; Humans; Intranuclear Space; Motor Neurons; Nuclear Proteins; Organelles; S Phase; *Transcription, Genetic; Zinc Fingers

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The zinc finger protein ZPR1 is present in both the cytoplasm and nucleoplasm. Cell cycle analysis demonstrates that ZPR1 undergoes major changes in subcellular distribution during proliferation. ZPR1 is diffusely localized throughout the cell during the G(1) and G(2)/M phases of the cell cycle. In contrast, ZPR1 redistributes to the nucleus during S phase and ZPR1 exhibits prominent co-localization with the survival motor neurons protein and the histone gene-specific transcription factor NPAT in subnuclear foci, including Cajal bodies that associate with histone gene clusters. ZPR1 deficiency causes disruption of survival motor neurons and NPAT localization within the nucleus, blocks S phase progression, and arrests cells in both the G(1) and G(2) phases of the cell cycle. These changes in subnuclear architecture and cell cycle progression may be caused by transcriptional defects in ZPR1-deficient cells, including decreased histone gene expression.

Rights and Permissions

Citation: J Biol Chem. 2006 Dec 29;281(52):40330-40. Epub 2006 Oct 26. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

The Journal of biological chemistry

PubMed ID

17068332