Title

Activation of recF-dependent recombination in Escherichia coli by bacteriophage lambda- and P22-encoded functions

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

9-1-1988

Document Type

Article

Subjects

Alleles; Bacterial Proteins; Bacteriophage lambda; *Conjugation, Genetic; Escherichia coli; Genes, Bacterial; Genes, Viral; Mutation; Plasmids; *Recombination, Genetic; Salmonella Phages

Disciplines

Life Sciences | Medicine and Health Sciences | Microbiology

Abstract

Escherichia coli strains bearing wild-type and mutant alleles of various recombination genes, as well as plasmids that express recombination-related genes of bacteriophages lambda and P22, were tested for their proficiency as recipients in Hfr-mediated conjugation. It was found that the homologous recombination systems of both phages could promote recombination in a recB recC mutant host. In addition, the Abc function of P22, but not the Gam function of lambda, was found to inhibit recombination in a wild-type host; however, both Abc and Gam inhibited recombination in a recF mutant host. These observations are interpreted as indicating that the recombination systems of both phages, as well as the RecBCD-modulating functions Abc and Gam, all activate the RecF recombination pathway of E. coli.

Rights and Permissions

Citation: J Bacteriol. 1988 Sep;170(9):4379-81. Link to article on publisher's website

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of bacteriology

PubMed ID

2842317