Title

Anaerobic induction of the alkylation-inducible Escherichia coli aidB gene involves genes of the cysteine biosynthetic pathway

UMMS Affiliation

Department of Molecular Genetics and Microbiology; Department of Cell Biology

Publication Date

3-1-1992

Document Type

Article

Subjects

Alkylation; Anaerobiosis; Cysteine; *DNA Repair; DNA Transposable Elements; Escherichia coli; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Suppressor

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The Escherichia coli aidB gene is a component of the adaptive response to alkylation damage. This gene is subject to two different forms of induction: an ada-dependent alkylation induction and an ada-independent induction that occurs when cells are grown anaerobically (M. R. Volkert, L. I. Hajec, and D. C. Nguyen, J. Bacteriol. 171:1196-1198, 1989; M. R. Volkert, and D. C. Nguyen, Proc. Natl. Acad. Sci. USA 81:4110-4114, 1984). In this study, we isolated and characterized strains bearing mutations that specifically affect the anaerobic induction pathway. This pathway requires a functional cysA operon, which encodes sulfate permease. Mutations in cysA block this pathway of aidB induction. In contrast, mutations in either cysH, cysD, cysN, or cysC result in elevated levels of aidB expression during aerobic growth. These results indicate that the sulfate transport genes perform a role in anaerobic induction of the aidB gene and suggest that growth under anaerobic conditions may modify either the function or the expression of gene products encoded by the cysA operon.

Rights and Permissions

Citation: J Bacteriol. 1992 Mar;174(6):2043-6.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of bacteriology

PubMed ID

1312537