Title

Properties of Escherichia coli expressing bacteriophage P22 Abc (anti-RecBCD) proteins, including inhibition of Chi activity

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Date

3-1-1993

Document Type

Article

Subjects

Bacteriophage P22; Base Sequence; Binding Sites; Conjugation, Genetic; DNA Helicases; DNA, Bacterial; Escherichia coli; effects; *Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases; Molecular Sequence Data; Nalidixic Acid; Plasmids; Recombination, Genetic; SOS Response (Genetics); Ultraviolet Rays

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Escherichia coli strains bearing plasmids expressing phage P22 anti-RecBCD functions abc1 and abc2 were tested for the presence of recBC-like phenotypes. Abc2 induces moderate sensitivity to UV light in wild-type and recD mutant strains but severely sensitizes both recF and recJ mutants. Abc1 has little effect on UV sensitivity in wild-type or recF or recJ mutant hosts but increases the sensitivity of recD mutants to a UV dose of 20 J/m2 about 10-fold. Abc2 induces E. coli to segregate inviable cells during growth, interferes with the growth of lambda red gam chi+ and chi 0 phage (the effect is greater with chi+ phage), inhibits Chi and Chi-like activity as measured by lambda red gam crosses, and prevents SOS induction in response to nalidixic acid; Abc1 has no effect in these tests. Abc2, alone or with Abc1, does not allow the growth of lambda red gam in the presence of a P2 prophage but does not kill the P2 lysogenic host (as lambda Gam does). Finally, Abc2 inhibits conjugational recombination in wild-type cells to the level seen in recBC mutants. These data suggest that Abc2 inhibits the recombination-promoting ability of RecBCD but leaves the exonuclease functions intact.

Rights and Permissions

Citation: J Bacteriol. 1993 Mar;175(6):1756-66.

Related Resources

Link to Article in PubMed

Journal Title

Journal of bacteriology

PubMed ID

8383665