Title

Induction of the Escherichia coli aidB gene under oxygen-limiting conditions requires a functional rpoS (katF) gene

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Date

12-1-1994

Document Type

Article

Subjects

Acetates; Alkylation; Alleles; Bacterial Proteins; DNA Mutational Analysis; DNA-Binding Proteins; Dose-Response Relationship, Drug; Escherichia coli; *Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Hydrogen Peroxide; Kanamycin Resistance; Mutagenesis, Insertional; Oxygen; Sigma Factor; Suppression, Genetic; Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The Escherichia coli aidB gene is regulated by two different mechanisms, an ada-dependent pathway triggered by methyl damage to DNA and an ada-independent pathway triggered when cells are grown without aeration. In this report we describe our search for mutations affecting the ada-independent aidB induction pathway. The mutant strain identified carries two mutations affecting aidB expression. These mutations are named abrB (aidB regulator) and abrD. The abrB mutation is presently poorly characterized because of instability of the phenotype it imparts. The second mutation, abrD1, reduces the expression of aidB observed when aeration is ceased and oxygen becomes limiting. Genetic and phenotypic analysis of the abrD1 mutation demonstrates that it is an allele of rpoS. Thus, aidB is a member of the family of genes that are transcribed by a sigma S-directed RNA polymerase holoenzyme. Examination of aidB expression in an rpoS insertion mutant strain indicates that both rpoS13::Tn10 and abrD1 mutations reduce aidB expression under oxygen-limiting conditions that prevail in unaerated cultures, reduce aidB induction by acetate at a low pH, but have little or no effect on the ada-dependent alkylation induction of aidB.

Rights and Permissions

Citation: J Bacteriol. 1994 Dec;176(24):7638-45.

Related Resources

Link to Article in PubMed

Journal Title

Journal of bacteriology

PubMed ID

8002588