Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12
Department of Molecular Genetics and Microbiology
Bacterial Proteins; Bacteriophage lambda; Chromosomes, Bacterial; Endodeoxyribonucleases; Escherichia coli; *Escherichia coli Proteins; Phenotype; Recombination, Genetic
Life Sciences | Medicine and Health Sciences
The rap gene of bacteriophage lambda was placed in the chromosome of an Escherichia coli K-12 strain in which the recBCD gene cluster had previously been replaced by the lambda red genes and in which the recG gene had been deleted. Recombination between linear double-stranded DNA molecules and the chromosome was tested in variants of the recGDelta red(+) rap(+) strain bearing mutations in genes known to affect recombination in other cellular pathways. The linear DNA was a 4-kb fragment containing the cat gene, with flanking lac sequences, released from an infecting phage chromosome by restriction enzyme cleavage in the cell. Replacement of wild-type lacZ with lacZ::cat was monitored by measuring the production of Lac-deficient chloramphenicol-resistant bacterial progeny. The results of these experiments indicated that the lambda rap gene could functionally substitute for the E. coli ruvC gene in Red-mediated recombination.
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Citation: J Bacteriol. 2002 Aug;184(16):4626-9.
Journal of bacteriology
Poteete, Anthony R.; Fenton, Anita C.; and Wang, Hsinju R., "Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12" (2002). Open Access Articles. 654.