Title

Modulation of DNA repair and recombination by the bacteriophage lambda Orf function in Escherichia coli K-12

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

4-20-2004

Document Type

Article

Subjects

Alleles; Bacteriophage lambda; *DNA Repair; Escherichia coli; Genes, Viral; *Recombination, Genetic; Ultraviolet Rays

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The orf gene of bacteriophage lambda, fused to a promoter, was placed in the galK locus of Escherichia coli K-12. Orf was found to suppress the recombination deficiency and sensitivity to UV radiation of mutants, in a Delta(recC ptr recB recD)::P(tac) gam bet exo pae cI DeltarecG background, lacking recF, recO, recR, ruvAB, and ruvC functions. It also suppressed defects of these mutants in establishing replication of a pSC101-related plasmid. Compared to orf, the recA803 allele had only small effects on recF, recO, and recR mutant phenotypes and no effect on a ruvAB mutant. In a fully wild-type background with respect to known recombination and repair functions, orf partially suppressed the UV sensitivity of ruvAB and ruvC mutants.

Rights and Permissions

Citation: J Bacteriol. 2004 May;186(9):2699-707.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of bacteriology

PubMed ID

15090511