Title

The HIV-1 Tat cellular coactivator Tat-SF1 is a general transcription elongation factor

UMMS Affiliation

Howard Hughes Medical Institute; Program in Molecular Medicine; Program in Gene Function and Expression

Publication Date

10-9-1998

Document Type

Article

Subjects

Electrophoresis, Polyacrylamide Gel; Gene Products, tat; HIV Long Terminal Repeat; *HIV-1; Humans; Plasmids; Trans-Activators; Transcription, Genetic; tat Gene Products, Human Immunodeficiency Virus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The human immunodeficiency virus type 1 (HIV-1) Tat protein strongly and specifically stimulates transcription elongation from the HIV-1 LTR and provides an important in vitro model system to study this process. Here we use protein-affinity chromatography to identify cellular factors involved in transcription elongation. A Tat-affinity column bound one transcription factor, Tat-SF1, efficiently and selectively. Tat-SF1 was identified originally as a Tat-specific coactivator, but we show it is a general transcription elongation factor. Our results also reveal the existence of an ATP-inactivatable general elongation factor (AIEF) required for Tat-SF1 activity and for which Tat can substitute functionally.

Rights and Permissions

Citation: Genes Dev. 1998 Oct 1;12(19):2992-6.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Genes and development

PubMed ID

9765201