Title

Cell cycle-regulated histone acetylation required for expression of the yeast HO gene

UMMS Affiliation

Program in Molecular Medicine and Department of Biochemistry and Molecular Biology

Publication Date

6-11-1999

Document Type

Article

Subjects

Acetylation; *Cell Cycle; *Cell Cycle Proteins; *DNA-Binding Proteins; Deoxyribonucleases, Type II Site-Specific; Fungal Proteins; G1 Phase; Gene Expression Regulation, Fungal; Genes, Fungal; Histone Deacetylases; Histones; Nucleosomes; Promoter Regions (Genetics); Protein Kinases; *Repressor Proteins; S Phase; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Expression of the yeast HO gene in late G1 of the cell cycle requires the SWI/SNF chromatin remodeling complex, the Gcn5p histone acetyltransferase, and two different sequence-specific transcriptional activators, Swi5p and Swi4p/Swi6p. We have used chromatin immunoprecipitation assays to investigate the role of each of these trans-acting factors in establishing a cell cycle-regulated domain of histone acetylation surrounding the HO upstream regulatory region. We detect a approximately 1-kb domain of H3 and H4 acetylation that is established in mid-G1, prior to and independent of HO transcription, which then declines with kinetics similar to inactivation of HO. This cell cycle burst of histone acetylation requires Gcn5p, SWI/SNF, and the Swi5p activator, but occurs in the absence of the Swi4p activator. We also find that inactivation of the Sin3p/Rpd3p deacetylase complex leads to a high level of acetylation at the HO locus throughout the cell cycle. We propose a sequential model for activation of HO in which the Swi5p-dependent recruitment of the Gcn5p acetyltransferase requires chromatin remodeling events by the SWI/SNF complex.

Rights and Permissions

Citation: Genes Dev. 1999 Jun 1;13(11):1412-21.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Genes and development

PubMed ID

10364158