The viral protein Apoptin associates with the anaphase-promoting complex to induce G2/M arrest and apoptosis in the absence of p53
Howard Hughes Medical Institute; Program in Molecular Medicine; Program in Gene Function and Expression
Apoptosis; Base Sequence; Capsid Proteins; DNA Primers; Fluorescent Antibody Technique; G2 Phase; Humans; Mitosis; Precipitin Tests; Protein Binding; Tumor Suppressor Protein p53; Ubiquitin-Protein Ligase Complexes
Life Sciences | Medicine and Health Sciences
The chicken anemia virus protein Apoptin induces apoptosis in the absence of p53 by a mechanism that remains to be elucidated. Here we show that in transformed cells, Apoptin is associated with APC1, a subunit of the anaphase-promoting complex/cyclosome (APC/C). We demonstrate that Apoptin expression, or depletion of APC1 by RNA interference, inhibits APC/C function in p53 null cells, resulting in G2/M arrest and apoptosis. Our results explain the ability of Apoptin to induce apoptosis in the absence of p53 and suggest that the APC/C is an attractive target for anticancer drug development.
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Citation: Genes Dev. 2004 Aug 15;18(16):1952-7. Link to article on publisher's site