Interplay between Ino80 and Swr1 chromatin remodeling enzymes regulates cell cycle checkpoint adaptation in response to DNA damage
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2006-09-05Keywords
Adaptation, PhysiologicalAdenosine Triphosphatases
Cell Cycle
Chromatin Assembly and Disassembly
DNA Damage
DNA Repair
Histones
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Ino80 and Swr1 are ATP-dependent chromatin remodeling enzymes that have been implicated in DNA repair. Here we show that Ino80 is required for cell cycle checkpoint adaptation in response to a persistent DNA double-strand break (DSB). The failure of cells lacking Ino80 to escape checkpoint arrest correlates with an inability to maintain high levels of histone H2AX phosphorylation and an increased incorporation of the Htz1p histone variant into chromatin surrounding the DSB. Inactivation of Swr1 eliminates this DNA damage-induced Htz1p incorporation and restores H2AX phosphorylation and checkpoint adaptation. We propose that Ino80 and Swr1 function antagonistically at chromatin surrounding a DSB, and that they regulate the incorporation of different histone H2A variants that can either promote or block cell cycle checkpoint adaptation.Source
Genes Dev. 2006 Sep 1;20(17):2437-49. Link to article on publisher's site
DOI
10.1101/gad.1440206Permanent Link to this Item
http://hdl.handle.net/20.500.14038/42203PubMed ID
16951256Related Resources
ae974a485f413a2113503eed53cd6c53
10.1101/gad.1440206